Roma are expected to make eight changes from the team that beat Bologna, with Henrikh Mkhitaryan and Edin Dzeko in the line-up against Ajax.
The match kicks off at 20.00 UK time.
After having returned from the first leg in Amsterdam with a 2-1 win, Roma must complete the job when the Dutch giants arrive in the Italian capital tonight.
After the 1-0 win over Bologna, the Giallorossi seem set to make several changes in the starting line-up for the most important match of the season.
Gianluca Mancini, Roger Ibanez and Amadou Diawara are tipped to keep their sport in the team, whilst Chris Smalling and Leonardo Spinazzola are still out.
Pau Lopez should start in goal against Ajax, with Bryan Cristante back in defence. Rick Karsdorp is expected at right and Riccardo Calafiori at left, with Jordan Veretout next to Diawara in the middle.
Coach Paulo Fonseca should lean on Lorenzo Pellegrini and Mkhitaryan to support Dzeko in attack.
Ajax should have former Roma goalkeeper Maarten Stekelenburg between the sticks, in the usual 4-3-3 of Erik ten Hag.
The guests attacking trident will most likely be composed of Antony, Dusan Tadic and David Neres.
Roma (probable): Pau Lopez; Mancini, Cristante, Ibanez; Karsdorp, Veretout, Diawara, Calafiori; Pellegrini, Mkhitaryan; Dzeko
Ajax (probable): Stekelenburg; Klaiber, Timber, Martinez, Tagliafico; Klaassen, Alvarez, Gravenberch; Antony, Tadic, Neres
Referee: Taylor (ENG)